↳ Grades 9-12: Life, Earth, and Social Sciences.↳ Grades 6-8: Math and Computer Science.↳ Grades 6-8: Life, Earth, and Social Sciences.↳ Grades K-5: Math and Computer Science.↳ Grades K-5: Life, Earth, and Social Sciences.Active Forums (Make all new posts here).Inoculate each plate with one loopful of urine #Gridded petri dish series#Remove a loopful of urine and inoculate each plate by making a straight line down the center and then a series of close perpendicular streaks throught the first line. Only move the loop straight up and downģ. Vertically insert a flamed and cooled calibrated loop into the specimen and immerse it just below the surface of the specimen. Use the following procedure to perform a quantitative culture of urine sing a calibrated loopĢ. If a 0.01 ml loop is used and 10 colonies are observed, the colony count would be 10 x 100 or 1000 CFU/ml (103 CFU/ml). For example, if a 0.001 ml loop is used and 10 colonies are observed, the colony count would be 10 x 10000 or 10,000 CFU/ml (104 CFU/ml). Each plate is inoculated with one loopful of urine, and the colony count is determined by multiplying the number of colonies by the dilution factor. The most common method uses a calibrated platinum loop that delivers 0.001 ml or 0.01 ml of urine. Quantitative cultures should be performed on all urine specimens so that the number of bacteria per milliliter of urine can be determined and expressed as CFU/ml. Counting colony usually does not require one to have any special lab equipment, here is a procedure in quantitative urine culture method which can be apply to other type of fluid.
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